It is reasonable to assume that female dogs have pain during estrus or their period. Your female dog will be restless, anxious, fretful, and uncomfortable, parallelling the human female. There is no definitive sign of pain. As so often suggested, if you spay your female puppy, you avoid ever having to wonder. This article will explore the female dogs estrus cycle and what to do if you believe your dog is in pain. Dogs and Their Period, aka, Estrus. There are many causes of pain for female ...